Regulatory

Part:BBa_K1628004

Designed by: Xinhao Song   Group: iGEM15_Nankai   (2015-08-04)

C2up

Promoter C2up is an artificially synthesized promoter. We have validate this part through promoter strength assay using bgaB (encoding β-galatosidase downstream of the multiple cloning sites) as reporter gene. According to promoter strength assay in B. amyloliquefaciens NK-1 (showed in Figure 1), C2up is the strongest promoters we used in our project.

Promoter4 NK.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None