Part:BBa_K1627008
Yellow Fluorescent Protein, with Medium Promoter and RBS
This is a plasmid using the pSB1C3 backbone, which is composed of the CamR resistance gene and the pMB1 origin of replication. A vector was inserted into the plasmid backbone,BBa_K608006 (J23110 + B0032), which is composed of the Bio-Brick promoter BBa_J23110 and ribosome binding site BBa_B0032. A fluorescent protein gene was then inserted into the resulting plasmid. BBa_K592101, the Yellow Fluorescent Protein gene was the fluorescent gene inserted in the plasmid.
This plasmid allows the creation of the yellow fluorescent protein inside bacteria.
This plasmid (BBa_K1627008) was used in the study of stability of genetic devices across generations by the 2015 University of Texas at Austin iGEM team. The plasmid has been observed be remain genetically stable across one-hundred generations. Point mutation in the promoter and IS element inserts were prominent mutations that destabilized the plasmid. Specifically, IS10R and IS10L was a common IS element inserted into the plasmid.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 706
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