Composite

Part:BBa_K1622001

Designed by: Alma Chapet--Batlle, Lucas Krauss   Group: iGEM15_Pasteur_Paris   (2015-09-18)


Coupled Esterase and Terephthalic acid transporter

This BioBrick is the assembly of : - BBa_K808007, coding for the NB-Esterase 13. This Enzyme catalyzes the cutting of PET into terephthalic acid (TPA) and ethylene glycol. - BBa_K808030, coding for the Terephthalic acid transporter. This enzyme enables the entry of TPA into our bacteria.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1792
    Illegal BglII site found at 2797
    Illegal BglII site found at 5550
    Illegal BamHI site found at 1144
    Illegal BamHI site found at 4284
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1428
    Illegal NgoMIV site found at 2037
    Illegal NgoMIV site found at 2421
    Illegal NgoMIV site found at 3610
    Illegal NgoMIV site found at 4042
    Illegal NgoMIV site found at 6076
    Illegal NgoMIV site found at 6214
    Illegal AgeI site found at 979
    Illegal AgeI site found at 3301
    Illegal AgeI site found at 5621
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3124
    Illegal SapI site found at 961


[edit]
Categories
Parameters
None