Coding

Part:BBa_K1622000

Designed by: Lucas Krauss   Group: iGEM15_Pasteur_Paris   (2015-09-18)


Glycoaldehyde reductase and dehydrogenase

Our part is the combination of two genes coding for enzymes that belong to the degradation pathway of Ethylene Glycol: - the Glycoaldehyde Reductase (BBa_K936023): this enzyme catalyzes the transformation of Ethylene Glycol into Glycoaldehyde. - the Glycoaldehyde Dehydrogenase (BBa_K936011): this enzyme catalyzes the reaction from Glycoaldehyde to Glycolate.

We used it as one of our clusters in the PET degradation operon.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2122
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 256
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 528
    Illegal AgeI site found at 2372
    Illegal AgeI site found at 2573
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None