Coding
SmtA

Part:BBa_K1620007

Designed by: Celio Dias Santos Jr   Group: iGEM15_UFSCar-Brasil   (2015-09-04)

Synechococcus metallothionenin A (SmtA)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 121
  • 1000
    COMPATIBLE WITH RFC[1000]

This biobrick is a improvement of biobrick BBa_K519010

Usage and Biology

SmtA is a metallothionein which bind to divalent metallic cations such as Zn(II). This SmtA was an improvement of the biobrick BBa_K519010 originally cloned from Synechococcus sp. PCC7942, a cyanobacterial strain. SmtA is a low cys content type II metallothionein. Other team (iGEM11_Tokyo-NoKoGen, 2011-09-30) have previously find SmtA constitutively expressed in E. coli improving its growth at a high Cd(II) concentrationwhen compared to wild type E. coli. This part was designed to eliminate an internal restriction site for PstI, the 98th base was changed G -> A. No residues were changed.

Figure 1: Improved SmtA (BBa_K1620007) compared to BBa_K519010; this new sequence is biobrick compatible due the removal of a PstI restriction site previously present in BBa_K519010.


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Categories
//cds
Parameters
None