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Regulatory
Zasp

Part:BBa_K1620001

Designed by: Celio Dias Santos Jr   Group: iGEM15_UFSCar-Brasil   (2015-09-03)

Promoter element Zasp

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Usage and Biology

It is a zinc activity sensing promoter element. The regulation of this promoter is Zur dependent and positive in absent of zinc or other divalent metallic cations in medium. The Zur protein binds those cations repressing the expression of the gene downstream Zasp element. In our project, it was used to trigger the expression of killer genes downstream this element. Since ZnuABC activity is extremely sensitive to low zinc concentrations, we believe that this component will be useful for other teams in order to its inducible feature and precise functioning.

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Categories
//promoter
Parameters
None