Coding
ProGlgBRv3

Part:BBa_K1618027

Designed by: Mark Elms Farhan Mitha   Group: iGEM15_NRP-UEA-Norwich   (2015-09-03)

GlgB-Rv3037c with IPTG-inducible promoter

K1618024 contains the coding sequences for GlgB from E. coli and the coding sequence for Rv3037c from Mycobacterium tuberculosis (strain ATCC 25618) under control of the LacI promoter. GlgB cleaves alpha-1,4 linkages in glycogen chains and transfers the cleaved branch onto the main glycogen chain using an alpha-1,6 linkage to make a branch point. Rv3037c is a putative S-adenosylmethionine-dependent methyltransferase and/or acyltransferase. This part will potentially make branched, acylated glycogen.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 283
    Illegal BamHI site found at 1491
    Illegal BamHI site found at 3142
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2952
    Illegal NgoMIV site found at 3125
    Illegal NgoMIV site found at 3449
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 917


[edit]
Categories
Parameters
None