Composite

Part:BBa_K1618020

Designed by: Kieran Rustage   Group: iGEM15_NRP-UEA-Norwich   (2015-08-28)

Plac-Buk-PTB

K1618020 contains the coding sequences for butyrate kinase and phosphotransbutrylase from Coprococcus sp. (L2-50 DSM 16842) under control of the LacI promoter. This part should catalyze the conversion of Butyrate to/from Butyryl-CoA though an intermediate of butyryl-phosphate 1.

1. Louis P., Petra L., Pauline Y., Grietje H., and Flint H.J., 2010, Diversity of human colonic butyrate-producing bacteria revealed by analysis of the butyryl-CoA:acetate CoA-transferase gene , Environmental Microbiology, 12(2) p. 304–14.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 508
    Illegal BglII site found at 748
    Illegal BglII site found at 889
    Illegal BglII site found at 1623
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1449


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Categories
Parameters
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