Coding

Part:BBa_K1618010

Designed by: Farhan Mitha   Group: iGEM15_NRP-UEA-Norwich   (2015-08-13)


pSB1C3-GlgX

GlgX is a genetic sequence which codes for a glycogen debranching protein. This protein reduces the amount of branching in glycogen strands resulting in glycogen which is far more linear. The genetic sequence is inserted into the standard iGEM vector.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 2211
    Illegal EcoRI site found at 1329
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 1329
    Illegal SpeI site found at 2212
    Illegal PstI site found at 2226
    Illegal NotI site found at 7
    Illegal NotI site found at 2219
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 1329
    Illegal BamHI site found at 77
    Illegal BamHI site found at 1285
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 2212
    Illegal EcoRI site found at 1329
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal EcoRI site found at 1329
    Illegal XbaI site found at 16
    Illegal SpeI site found at 2212
    Illegal PstI site found at 2226
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 711


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Parameters
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