Part:BBa_K1616009

HokD reversed - RBS and double T7 terminator

HokD toxin

The HokD protein is a toxin when overexpressed kills the cells from the inside by interfering with a vital function in the cell membrane. It has been shown that the hokD gene can be used to design efficient suicide functions to contain bacterial growth (Knudsen and Karlstrom 1991, Knudsen, Saadbye et al. 1995)

Reverse sequence

This composite part is composed of the reverse sequence of HokD with reverse RBS (BBa_K1616011) and the double terminator T7 reversed (BBa_B0025). This part has been created in order to work with a reversed promoter, which will allow the synthesized of HokD toxin.

Fig. 1: HokD reversed

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

All BioBrick parts used for assembling the composite part are compatible with the RFC10 Biobrick standard.

Source

This composition part is composed of reversed sequence of the HokD from BBa_K1616008, and RBS reversed BBa_K1616011 and the double terminator T7 reversed BBa_B0025.

References

Knudsen, S., P. Saadbye, L. H. Hansen, A. Collier, B. L. Jacobsen, J. Schlundt and O. H. Karlstrom (1995). "Development and testing of improved suicide functions for biological containment of bacteria." Appl Environ Microbiol 61(3): 985-991.