Composite
Part:BBa_K1615088
Designed by: Brooke Rothschild-Mancinelli Group: iGEM15_Edinburgh (2015-09-16)
TVEL5 laccase fused to Linker-CBDcipA-linker driven by LacI promoter
TVEL5 laccase fused N terminally to CBDcipA with N and C terminal linkers (BBa_K1615078) driven by the IPTG inducible PLlac 0-1 promoter (BBa_K314103). This allows the TVEL5 laccase CBD fusion to express when induced with IPTG.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2265
Illegal BglII site found at 2426
Illegal BamHI site found at 2489 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 126
Illegal NgoMIV site found at 1648 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 2071
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Categories
Parameters
None |