Composite

Part:BBa_K1615081

Designed by: Brooke Rothschild-Mancinelli   Group: iGEM15_Edinburgh   (2015-09-02)


Linker+dCBD fused to TVEL5 driven by LacI promoter

TVEL5 Laccase fused C terminally to dCBD + N-terminal linker driven by the IPTG inducible PLlac 0-1 promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2653
    Illegal BglII site found at 2814
    Illegal BamHI site found at 2877
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 126
    Illegal NgoMIV site found at 1656
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2459


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Categories
Parameters
None