Composite

Part:BBa_K1615079

Designed by: Brooke Rothschild-Mancinelli   Group: iGEM15_Edinburgh   (2015-08-28)

CBDcenA+Linker fused to TVEL5 driven by LacI promoter

CBDcenA+Linker fused to TVEL5 Laccase (BBa_K1615069) driven by LacI promoter (BBa_K314103). This allows the TVEL5 laccase CBD fusion to express when induced with IPTG.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2679
    Illegal BglII site found at 2840
    Illegal BamHI site found at 2903
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 126
    Illegal NgoMIV site found at 1648
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2485


[edit]
Categories
Parameters
None