Composite

Part:BBa_K1615058

Designed by: Brooke Rothschild-Mancinelli   Group: iGEM15_Edinburgh   (2015-09-02)


Heroin esterase fused to CBDcenA+Linker in RFC25 driven by LacI promoter

Heroin Esterase fused N terminally to CBDcenA + C terminal linker driven by the IPTG inducible PLlac 0-1 promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1665
    Illegal BamHI site found at 2421
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 126
    Illegal NgoMIV site found at 2623
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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