Composite

Part:BBa_K1615057

Designed by: Brooke Rothschild-Mancinelli   Group: iGEM15_Edinburgh   (2015-09-02)


CBDcenA+Linker fused to Heroin Esterase driven by LacI promoter

Heroin Esterase fused C terminally to CBDcenA + C-terminal linker driven by the IPTG inducible PLlac 0-1 promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2097
    Illegal BamHI site found at 2853
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 126
    Illegal NgoMIV site found at 1648
    Illegal NgoMIV site found at 2071
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None