Composite
Part:BBa_K1615014
Designed by: Brooke Rothschild-Mancinelli Group: iGEM15_Edinburgh (2015-08-27)
Linker+dCBD fused to MorA driven by LacI promoter
Morphine-6-dehydrogenase fused to the C terminal of dCBD + N-terminal linker (BBa_K1615004) driven by the IPTG inducible PLlac 0-1 promoter (BBa_K314103). This allows the morphine-6-dehydrogenase CBD fusion to express when induced with IPTG.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 2865
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 126
Illegal NgoMIV site found at 1648 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |