Part:BBa_K1613018
Device with CopA promoter to detect copper
This is a device to detect copper. We use the CopA Promoter to detect (Promoter from BBa_K1555000) because it’s more specific and sensitive than MarO, CueO. We linked it with RBS, RFP, and Terminator.
Why do we detect Copper?
Copper is a compulsory ingredient in respiratory pigment, and have been identified in many other proteins and enzymes. In 1847, Harless found that copper has a great action in Molluscs, and in 1878 Frederig first separated copper from protein contamination in blood of octopus, and he named it as ceraloplasim. In 1928, Hart’s report said copper is a compulsory element.
According to the study, since copper can effect the absorption of iron, lack of copper will cause neutropenia, osteoporosis and growth retardation, or damage human’s immune system.
The harm of Copper
Too much copper harms human body. Inhale of high concentration of copper will result in irritation of the nose and throat. Ingesting high concentration of copper also lead to liver and kidney damage. Excess copper causes toxicity and the presenting symptoms include nausea, queasiness, vomit and dirrahea. Other severe symptoms like hematuria, jaundice, and urine oligonucleotide may appear too. Copper poisoning could lead to hemolysis, decrease of hemoglobin and increase of serum lactate dehydrogenase.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 882
Illegal AgeI site found at 994 - 1000COMPATIBLE WITH RFC[1000]
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