Part:BBa_K1613017

K1613017 is a new part which can detect lead (Pb) ions in the liquid.

This part is desinged to proceed lead detection in e.coli. When lead ions (Pb2+) enter the e.coli, they will combine with the protein PbrR and then form a dimer. The lead promoter can be controlled by the dimer to further trigger the RFP. The idea of this design came from Brown 2007 igem team, also working on lead detection. We combined I721001 and I721002 with a constitutive promoter J23102 and RFP to make them function.

Whether lead can enter e.coli or not: By using gold nanoparticles(AU-NPs), we measured the lead absorption of e.coli. The results showed that the longer e.coli put in Pb2+ or the higher Pb2+ concentration, the more lead entered the e.coli.

USE AND BIOLOGY
iGEM16_HSiTAIWAN: Measure fluorescence of lead detector

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 1281
  Illegal AgeI site found at 1393
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 686