Part:BBa_K1613010
A composite part that can detect the recA.
Introduction
The SOS response is a global response to DNA damage in which the cell cycle is arrested and DNA repair and mutagenesis are induced. The system involves the RecA protein (Rad51 in eukaryotes). The RecA protein, stimulated by single-stranded DNA, is involved in the inactivation of the LexA repressor thereby inducing the response. It is an error-prone repair system that is attributed to mutagenesis.
Design
The toxins will cause DNA damage, making DNA become single-strand DNA(ssDNA), which can activate protein RecA. So, what can protein RecA do? E.coli has a sos response system, and there is a repressor LexA which inhibits the next reaction, so the fluorescent gene won’t express. But when the a ctivated protein RecA comes and hydrolysis the repressor LexA, the next reaction ca n work, so the fluorescent gene will express. Therefore, we can know that there is Aflat oxin in the oil we test. These are all about our treatment group.
References
[1]ZHENG Lili, ZHU Yujing, SHAO Caimei, ZHANG Yong The Relationship between Cytochrome CYP3A4 in Vivo and the Toxicity of Aflatoxin B1 in Feedstuffs [2]Nejc Paulič2,†,Adrijana Leonardi1, Vesna Hodnik2,Gregor Anderluh2,3, Zdravko Podlesek2, Darja Žgur-Bertok2,Igor Križaj1,4,5 Matej Butala2. Structural insight into LexA–RecA* interaction Lidija Kovačič1
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 862
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