Part:BBa_K1613004

To detect toxicant harmful to human liver

Cytochrome P450 1A2 is a member of the cytochrome P450 mixed-function oxidase system. And it is involved in the metabolism of xenobiotics in the body.

Introduction

CYP1A2 can oxidize aflatoxin B1.

cyp1a2 can oxidize the substances that it can metabolism.

Cytochrome P450 1A2 is a member of the cytochrome P450 mixed-function oxidase system. And it is involved in the metabolism of xenobiotics in the body. Function The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. CYP1A2 is able to metabolize some PAHs to carcinogenic intermediates. Other xenobiotic substrates for this enzyme include aflatoxin B1, and acetaminophen.

CYP1A2 Substrates

many antidepressants amitriptyline clomipramine, some atypical antipsychotics clozapine olanzapine, ropivacaine, theophylline, zolmitriptan, melatonin, tamoxifen, erlotinib, cyclobenzaprine, estradiol, fluvoxaminemexiletine, naproxen, ondansetron, phenacetin, paracetamol, propranolol, riluzole, tacrine, tizanidine, verapamil, warfarin

Design

The results are verefied by the electrophoresis.

When aflatoxin B1 goes into the e.coli, CYP1A2 will oxidize the Aflatoxin B1, and then the aflatoxin B1 will turn into Aflatoxin oxidation AFBO.

We try three different RBS(B0030,B0034,B0032) to increase the protein expression.

Sources

NCBI, GeneBank:NM000761

References

1. Veronica S. Mary a, Ana Valdehita b, Jose M. Navas b, Hector R. Rubinstein a, Maria L. Fernandez-Cruz. Effects of aflatoxin B1, fumonisin B1 and their mixture on the aryl hydrocarbon receptor and cytochrome P450 1A induction
2. Yan-Ping Du Hui-Lian Zhu. The possible mechanism of Aflatoxin B1 Hepatocarcinogenesis and preventive effects of phytochemicals
3. Food hygiene standards - Food in aflatoxin limits (human) 82 1.4.4 Health Department Announcement fresh word No. 8189322
4. EU animal and plant inspection and quarantine of food hygiene regulations cum Profile
5. Veronica S. Mary a, Ana Valdehita b, Jose M. Navas b, Hector R. Rubinstein a,*Maria L. Fernandez-Cruz b. Effects of aflatoxin B1, fumonisin B1 and their mixture on the aryl hydrocarbon receptor and cytochrome P450 1A induction
6. ZHENG Lili, ZHU Yujing, SHAO Caimei, ZHANG Yong The Relationship between Cytochrome CYP3A4 in Vivo and the Toxicity of Aflatoxin B1 in Feedstuffs
7. Nejc Paulič2,†,Adrijana Leonardi1, Vesna Hodnik2,Gregor Anderluh2,3, Zdravko Podlesek2, Darja Žgur-Bertok2,Igor Križaj1,4,5 Matej Butala2. Structural insight into LexA–RecA* interaction Lidija Kovačič1

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1400
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 320
  Illegal NgoMIV site found at 407
  Illegal AgeI site found at 390
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 1120