Intermediate

Part:BBa_K1613001

Designed by: Ko-yu Chang   Group: iGEM15_HSNU-TAIPEI   (2015-09-05)

J23119:B0032

We use the promoter to control cyp1a2.

Introduction

J23119+B0032+E0040+B0015

A promoter which can be actiated by RecA protein. We use recA promoter to control the reporter gene, because it can be activated by recA protein which is activated by ssDNA. So when E.coli,s sos response system works, the fluorescent gene can be expressed.

References

  1. Nejc Paulič2,†,Adrijana Leonardi1, Vesna Hodnik2,Gregor Anderluh2,3, Zdravko Podlesek2, Darja Žgur-Bertok2,Igor Križaj1,4,5 Matej Butala2. Structural insight into LexA–RecA* interaction Lidija Kovačič1
  2. ROGER BRENT AND MARK PTASHNE Department ofBiochemistry and Molecular Biology, Harvard University, Cambridge, Massachusetts 02138 Contributed by Mark Ptashne, April 9, 1981. Mechanism of action of the lexA gene product

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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