Coding
Part:BBa_K1611001
Designed by: Frederic Ros Group: iGEM15_Evry (2015-09-15)
MATalpha-IFNgamma
Murine IFN gamma cytokine codon optimized to give the best yield in yeast S. cerevisiae. It promotes T cell activation, upregulates MHC-I and is pro-inflammatory. MAT alpha is the peptide leader used as a secretion signal and fused to IFN gamma without ATG.
Part sequencing
Figure 1: Sequencing of MATalpha-IFNgamma cloned in pSB1C3 vector. The black line shows the consensus sequence.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 244
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 297
[edit]
Categories
Parameters
//chassis/eukaryote/yeast
None |