Composite

Part:BBa_K1610404

Designed by: Leon Yim   Group: iGEM15_TAS_Taipei   (2015-09-18)


pDAPa + RBS + T4 Endo + Term

This part is a safety circuit containing repressible pDAPa promoter, a strong RBS, T4 Endolysin, and a terminator. When this construct is in E. coli with no dap acid to repress pDAPa promoter, then the promoter activates and produces the downstream T4 Endolysin which is a lysozyme capable of degrading the E. coli peptidoglycan membrane layer.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 380
    Illegal AgeI site found at 450
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None