Coding

Part:BBa_K1610201

Designed by: Leon Yim   Group: iGEM15_TAS_Taipei   (2015-09-06)


human Antichymotrypsin with mutations for 3 amino acid changes (ACT3m)

This part is similar to human Antichymotrypsin (ACT) (Bba_K1610200) except it contains selected mutations. The mutations were made in order to allow ACT3m to bind and inhibit Granzyme B, a human enzyme that is found in the extracellular matrix, extracellularly.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 579
    Illegal XhoI site found at 954
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 880


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Categories
Parameters
None