Composite

Part:BBa_K1610104

Designed by: Leon Yim   Group: iGEM15_TAS_Taipei   (2015-09-06)


pTS + RBS + ACT3m + Term

This part consists of temperature sensitive promoter (Bba_K608351), a RBS (Bba_B0034), a coding sequence (Bba_K1610201), and a terminator (Bba_B0015). This part is used to produce a modified human antichymotrypsin, a human extracellular protein that has been given the ability to bind and inhibit Granzyme B. This circuit is temperature regulated, where environments at or above 37C will activate the promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NotI site found at 781
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1553
    Illegal XhoI site found at 1928
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1854


[edit]
Categories
Parameters
None