Composite

Part:BBa_K1610103

Designed by: Leon Yim   Group: iGEM15_TAS_Taipei   (2015-09-06)

pTS + RBS + ACT + Term

This part consists of temperature sensitive promoter (Bba_K608351), a RBS (Bba_B0034), a coding sequence (Bba_K1610200), and a terminator (Bba_B0015). This part is used to produce human antichymotrypsin, a human extracellular protein. This circuit is temperature regulated, where environments at or above 37C will activate the promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NotI site found at 781
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1553
    Illegal XhoI site found at 1928
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1854


[edit]
Categories
Parameters
None