Composite

Part:BBa_K1610102

Designed by: Leon Yim   Group: iGEM15_TAS_Taipei   (2015-09-06)


pTS + RBS + GFP + Term

This part consists of temperature sensitive promoter (Bba_K608351), a RBS (Bba_B0034), a coding sequence/reporter (Bba_E0040), and a terminator (Bba_B0015). This part is used to test the functionality of the temperature sensitive promoter. When grown at a variety of temperatures, it is observed that GFP is only found at 37C or above.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NotI site found at 781
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1618


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Categories
Parameters
None