Composite

Part:BBa_K1610101

Designed by: Leon Yim   Group: iGEM15_TAS_Taipei   (2015-09-06)


pTS + RBS

This party consists of a temperature sensitive promoter (Bba_K608351) and a RBS (Bba_B0034). The temperature sensitive promoter activates at and above 37 degrees Celsius. The addition of the RBS behind the temperature sensitive promoter means an ORF can be easily inserted downstream of this part and activated in a high temperature environment at and above 37C.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NotI site found at 781
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None