Composite

Part:BBa_K1608003

Designed by: Ching-Yuan Chen, Yi-Ru Liu, Pei-Hong Chen   Group: iGEM15_Mingdao   (2015-09-10)

SRPK-His/pSB1C3

In order to express SR (BBa_K1608000) and SRPK (BBa_K1608001) gene more conveniently, we made two composite biobricks with promoter, RBS and a fusion tag.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 97
    Illegal PstI site found at 926
    Illegal PstI site found at 977
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 926
    Illegal PstI site found at 977
    Illegal NotI site found at 2221
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 189
    Illegal BglII site found at 450
    Illegal XhoI site found at 1910
    Illegal XhoI site found at 2230
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 97
    Illegal PstI site found at 926
    Illegal PstI site found at 977
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 97
    Illegal PstI site found at 926
    Illegal PstI site found at 977
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2084


[edit]
Categories
Parameters
None