Coding

Part:BBa_K1608001

Designed by: Ching-Yuan Chen, Yi-Ru Liu, Pei-Hong Chen   Group: iGEM15_Mingdao   (2015-09-10)

SRPK/pSB1C3

SRSF protein kinase 1 (SRPK1) is a serine/arginine-rich protein specific kinase, which phosphorylates SR proteins on serine residues in SR domain. It plays an important role in the regulation of SR protein activity. Disorders in SRPK1 may be observed in certain cancer cells.

In [http://2015.igem.org/Team:Mingdao our project] in iGEM Mingdao 2015, we use it to phosphorylate SR protein (BBa_K1608000) to be a fire retardant protein.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 695
    Illegal PstI site found at 746
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 695
    Illegal PstI site found at 746
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 219
    Illegal XhoI site found at 1679
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 695
    Illegal PstI site found at 746
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 695
    Illegal PstI site found at 746
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1853


[edit]
Categories
Parameters
None