Coding

Part:BBa_K1607002

Designed by: Jiazheng Xing   Group: iGEM15_Shenzhen_SFLS   (2015-08-06)


HER2-extracellular domain mutant 1

Genetically modified HER2-ECD. This mutant has lower affinity with antibody cha21 than original HER2-ECD does.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 157
    Illegal PstI site found at 223
    Illegal PstI site found at 355
    Illegal PstI site found at 1527
    Illegal PstI site found at 1576
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 157
    Illegal PstI site found at 223
    Illegal PstI site found at 355
    Illegal PstI site found at 1527
    Illegal PstI site found at 1576
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 377
    Illegal BglII site found at 1043
    Illegal BamHI site found at 862
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 157
    Illegal PstI site found at 223
    Illegal PstI site found at 355
    Illegal PstI site found at 1527
    Illegal PstI site found at 1576
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 157
    Illegal PstI site found at 223
    Illegal PstI site found at 355
    Illegal PstI site found at 1527
    Illegal PstI site found at 1576
    Illegal NgoMIV site found at 661
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 55
    Illegal BsaI.rc site found at 296


[edit]
Categories
Parameters
None