Part:BBa_K1600000

EIIBC

This part serves to increase the uptake of glucose by the cell. It is designed to work in conjunction with EIIA (BBa_K1508003) or the composite EIIA device (BBa_K1600001), another part of glucose uptake in E. coli. EIIBC, also known as ptsG, is the active channel for glucose uptake in E. coli as a part of the Phosphotransferase System (PTS) so this BioBrick would upregulate the uptake of glucose. While EIIA is not a transmembrane transferase protein, it plays a prominent second step in the PTS pathway (http://spin.niddk.nih.gov/clore/Structures/HPr-IIAMtl/pict.html), the first step being glucose uptake by the transmembrane protein complex EIIBC. We hypothesize that the use of EIIBC with EIIA will increase glucose upake in E. coli.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 1312
  Illegal AgeI site found at 880
  
• 1000
  COMPATIBLE WITH RFC[1000]