Part:BBa_K1598007
J23101 promoter+RBS+FDXR+RBS+FDX1+ RBS + CYP11A1 gene+rrnb double terminator
Also called Cytochrome P450scc, Cholesterol Side Chain Cleavage Enzyme (P450scc) is a mitochondrial enzyme that converts cholesterol into pregnenolone, which is a neurosteroid as well as the precursor to a variety of steroid hormones and neurotransmitters. The gene that encodes it is CYP11A1. Furthermore, the catalysis involves 3 monooxygenase reactions, which requires two electron transport proteins: ferrodoxin (FDX1) and ferredoxin reductase (FDXR). Our biobrick consists of the iGEM prefix followed by the J23101 constitutive promoter, RBS, FDXR gene, RBS, FDX1 GENE, RBS, CYP11A1 gene, rrnb double terminator, and the iGEM suffix. This will be ligated onto a PSB1C3 backbone.
Emerging preclinical and clinical evidence suggests that pregnenolone may be a promising novel therapeutic candidate in schizophrenia. It has also been shown to reduce the side effects of benzodiazepines. Furthermore, cholesterol side chain cleavage is the 1st step in the biosynthesis of steroids such as testosterone, progesterone, cortisol, estrogen, etc. Therefore, this biobrick has a plethora of applications, and is in a way several biobricks in 1.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 25
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 25
Illegal NheI site found at 3264
Illegal NotI site found at 31 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 25
Illegal XhoI site found at 554
Illegal XhoI site found at 1446 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 25
- 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 25
Illegal XbaI site found at 40
Illegal NgoMIV site found at 1642
Illegal AgeI site found at 1079 - 1000COMPATIBLE WITH RFC[1000]
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