Part:BBa_K1590000

Human Haemoglobin A

A synthetic coding sequence for human Haemoglobin A. The protein forms part of the haemoglobin tetramer consisting of two A-chains and two B-chains (see BBa_K1590001 for Haemoglobin B). This biobrick is a synthetic sequence codon optimized for expression in an Escherichia coli chassis.

Usage and Biology

Haemoglobin is the tetrameric protein molecule in red blood cells that carries oxygen. It is composed of four polypeptide chains, which in adults consist of two alpha (A) globin chains and two beta (B) globin chains.

• iGEM Dundee 2015

This synthetic gene sequence was found to be correct. Unfortunately, attempts to show stable production of a protein product from this synthetic gene failed.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 342
  
• 1000
  COMPATIBLE WITH RFC[1000]