DNA
Part:BBa_K1588009
Designed by: Juan David Sifontes Group: iGEM15_UNA_Honduras (2015-09-18)
Protein with epitopes from dengue virus serotype 4 to generate immune response
it is a gene coding a modified Chitin Oligosaccharide Binding Protein (code 1ZTY) from Vibrio cholerae, we cut some residues from external loops and inserted human epitopes from serotype 4 of dengue virus instead, it is intended to generate an immune response against all serotypes of the virus, those epitopes come from T lymphocytes, it could be improved inserting epitopes from B lymphocytes
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 1279
Illegal PstI site found at 1432 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 1279
Illegal PstI site found at 1432 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 1279
Illegal PstI site found at 1432 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 1279
Illegal PstI site found at 1432
Illegal NgoMIV site found at 859
Illegal NgoMIV site found at 1690 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 938
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Categories
Parameters
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