DNA

Part:BBa_K1588005

Designed by: Juan David Sifontes   Group: iGEM15_UNA_Honduras   (2015-09-18)


tetravalent protein with epitopes from dengue virus to generate immune response

it is a gene coding a modified Chitin Oligosaccharide Binding Protein (code 1ZTY) from Vibrio cholerae, we cut some residues from external loops and inserted human epitopes from the 4 serotypes of dengue virus instead, it is intended to generate an immune response against all serotypes of the virus, those epitopes come from T lymphocytes, it could be improved inserting epitopes from B lymphocytes

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1210
    Illegal PstI site found at 1363
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1210
    Illegal PstI site found at 1363
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1210
    Illegal PstI site found at 1363
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1210
    Illegal PstI site found at 1363
    Illegal NgoMIV site found at 811
    Illegal NgoMIV site found at 1621
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 890


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Categories
Parameters
None