Device

Part:BBa_K1587009

Designed by: Alexandre Le Scornet   Group: iGEM15_Toulouse   (2015-09-16)

PompC and lacI box regulated cI and formate production

Uses a positively regulated promoter (PompC) and a lacI binding site to regulate the expression of cI. This construction was designed to be regulated by Cph8 red light receptor (Cph1-EnvZ chimeric protein). The purpose was to create a circadian switch to alternate the expression of two sets of genes (formate/butyrate).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1318
    Illegal BamHI site found at 3720
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1427
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3908


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