Coding

Part:BBa_K1585300

Designed by: Michael Osthege   Group: iGEM15_Aachen   (2015-08-23)

Glycogen synthase GlgA from E. coli K12

The glycogen synthase synthesizes alpha-1,4-glucan chains using ADP-glucose.

The BioBrick was created from the genomic sequence of glgA in E. coli K12.

Six illegal restriction sites were removed to ensure full BioBrick compability.

Usage and Biology

BBa_K1585300 was combined with the well-characterized RBS B0034 (see BBa_K1585310).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 916


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