Composite

Part:BBa_K1585212

Designed by: Laura Grabowski   Group: iGEM15_Aachen   (2015-08-25)

B0034.PHI

This is the translational unit of the 6-phospho-3-hexuloisomerase (Phi) from Bacillus methanolicus MGA3. It is codon optimized for E. coli.


Catalysed reaction by Phi
D-arabino-hex-3-ulose 6-phosphate → D-fructose 6-phosphate.


Usage and Biology

Aachen 15-08-10 Mdh, Hps, Phi f. Wiki 2.png

Mdh, Hps and Phi expressed in a pSB1A30 backbone on SDS-PAGE after IPTG induction with a RFP expression strain as a negative control. All genes expressed in BL21 Gold (DE3), samples taken from 50 ml main culture in shake flasks after 6 h and 21 h after IPTG induction.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None