Reporter

Part:BBa_K1584026

Designed by: All of us   Group: iGEM15_Elan_Vital_Korea   (2015-08-31)


Constitutive LuxR with AHL-inducible GFP

Part BBa_K1584026 is a reporter part that releases green fluorescent light. It produces two proteins, first LuxR and GFP. LuxR is constantly produced with constitutive promoter J61100. When AHL is present, it binds to LuxR. The resulting compound binds to the LuxRP promoter, producing green fluorescent proteins.

It includes Parts J61100, a ribosome binding site with a constitutive promoter from Part J23100 inputted, to promote the production of LuxR, C0062. The terminator behind LuxR was from Part K83017. Part J37032 was used as a GFP DNA segment controlled by the LuxRP promoter, to produce GFP.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 908
    Illegal BsaI.rc site found at 1635


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Parameters
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