Part:BBa_K1582018

LC+inJanus Fusion Protein

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 600
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 1075
  

Usage

This fusion protein is designed to degrade PET into terephthalic acid and ethylene glycol more powerfully. Compared to single LC, the rate of PET emzymolysis is supposed to be improved by LC-Janus fusion.

Biology

LC is a kind of cutinase, the details of which can be seen at BBa_K936000. Hydrophobin inJanus, from the edible mushroom Grifola frondosa, play a role in a broad range of processes in the growth and development of filamentous fungi. Their assembly could show rod-like structure. They can be expressed in eucaryotic cells.
According to some research, when Janus is added to the reaction of cutinase and plastics, the reaction rate is supposed to have an obvious improvement.
Based on the above background, we design to use the method of fusion protein to combine cutinase and Janus compactly, through the establishment of fusion protein, we can found if we can further promote the stimulation rate of the plastics degradation. Meanwhile, from the data about fusion protein, we can try to uncover the functional mechanism of the stimulation to the plastics degradation which is unknown until now.

Reference

[1]Zefang Wang, Shuren Feng, Yujian Huang, Shan Li, Haijin Xu, Xiuming Zhang, Yanling Bai, Mingqiang Qiao, Expression and characterization of a Grifola frondosa hydrophobin in Pichia pastoris, Protein Expression and Purification 72 (2010) 19–25.
[2]Doris Ribitsch, Enrique Herrero Acero, Agnes Przylucka, Enhanced cutinase-catalyzed hydrolysis of polyethylene terephthalate 2 by covalent fusion to hydrophobins, Appl. Environ. Microbiol. doi:10.1128/AEM.04111-14.