Part:BBa_K1565002
ColiAlert
This part is responsible for the pink coloration of E. coli. E. coli transformed with this biobrick becomes pink in neutral and basic medium (LB culture medium for example). However, cultures can change color to yellow (instantly) with the acidification of the medium. Another factor that makes the medium yellow, is the addition of cell lysis buffer. The cells lysed immediately becomes yellow, while those that are not lysed remain pink. Thus, this biobrick can be used as an indicator of cell lysis. All parts of the composite were developed from the iGEM registry. The chromoproteins used were submitted by the team of Uppsala in 2011. On the experience page there are the experiments that demonstrate the operation of the composite developed.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 401
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 778
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