Composite
ColiAlert

Part:BBa_K1565001

Designed by: Deborah M Landim Zanforlin   Group: iGEM14_LIKA-CESAR-BR   (2014-10-10)


Colorimetric indicator of cell lysis

This biobrick makes E. coli to have pink color under natural conditions. It is formed by biobricks already available in the registry. It is responsible for the production of yellow chromoprotein (BBa_K1033910) which in the presence of basic media change their color to pink. This part can be used as a reporter cell lysis.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1468
    Illegal BamHI site found at 1002
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 435
    Illegal NgoMIV site found at 891
    Illegal NgoMIV site found at 1878
    Illegal AgeI site found at 1155
    Illegal AgeI site found at 2514
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1152
    Illegal SapI.rc site found at 2891


[edit]
Categories
Parameters
None