DNA
Part:BBa_K1560004
Designed by: Chris Lee, Charles Paine, Max Genetti, Jeff Nivala, David Bernick Group: iGEM14_UCSC (2014-10-07)
Knock-In Knock-Out Vector for HVO_1140
Use this plasmid to perform pop in pop out deletion of acd2 in Haloferax Volcanii.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 737
Illegal NgoMIV site found at 947
Illegal NgoMIV site found at 1496 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 111
Illegal BsaI site found at 249
Illegal BsaI site found at 1019
Illegal BsaI site found at 1926
Illegal BsaI.rc site found at 1685
[edit]
Categories
Parameters
None |