DNA

Part:BBa_K1560004

Designed by: Chris Lee, Charles Paine, Max Genetti, Jeff Nivala, David Bernick   Group: iGEM14_UCSC   (2014-10-07)


Knock-In Knock-Out Vector for HVO_1140

Use this plasmid to perform pop in pop out deletion of acd2 in Haloferax Volcanii.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 737
    Illegal NgoMIV site found at 947
    Illegal NgoMIV site found at 1496
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 111
    Illegal BsaI site found at 249
    Illegal BsaI site found at 1019
    Illegal BsaI site found at 1926
    Illegal BsaI.rc site found at 1685


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Categories
Parameters
None