Plasmid_Backbone

Part:BBa_K1559011

Designed by: Xiuqi (Rex) Xia   Group: iGEM14_Toronto   (2014-10-14)

Low-copy plasmid pTRC99A (trc promoter and lacI removed)

pTRC99A converted to biobrick format, with the trc promoter and lacI removed.

Usage and Biology

This is a low-copy plasmid with ampicillin resistance. The original promoter that came with the plasmid was removed so you can use your own promoters.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2563
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
    Illegal NotI site found at 9
    Illegal NotI site found at 2569
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2563
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 2563
    Illegal suffix found at 2
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 2563
    Plasmid lacks a suffix.
    Illegal XbaI site found at 2578
    Illegal SpeI site found at 2
    Illegal PstI site found at 16
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 156
    Illegal BsaI site found at 1224
    Illegal SapI.rc site found at 2306


[edit]
Categories
Parameters
None