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Part:BBa_K1559009

Designed by: Xiuqi (Rex) Xia   Group: iGEM14_Toronto   (2014-10-13)

Randomized protospacer for CRISPR/Cas9 System

This is a randomly-generated 20bp sequence that does not occur anywhere in the E. coli MG1655 genome. It can be used as a protospacer (target site for Cas9 nuclease).

Usage and Biology

The protospacer and PAM forms the target site for the Cas9 nuclease. To make Cas9 target this site, the protospacer (not including the PAM) must be inserted into a crRNA array. (see Part:BBa_K1559002)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None