Generator

Part:BBa_K1559004

Designed by: Xiuqi (Rex) Xia   Group: iGEM14_Toronto   (2014-10-13)

CRISPR/Cas9 system with Anderson medium-expression constitutive promoter

CRISPR/Cas9 system driven by the Anderson medium-expression constitutive promoter. Please see Part:BBa_K1559002 for more details.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 1146
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3425
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4367
    Illegal BsaI.rc site found at 4344


[edit]
Categories
//function/crispr
//function/crispr/cas9
Parameters
None