RNA

Part:BBa_K1555006

Designed by: Xu Xuan   Group: iGEM14_NJAU_China   (2014-10-15)

sgRNA which cooperates with dCAS9 protein can target copa's CDS specifically

This sgRNA is specially designed for our copper sensor "copa" and it can target on the genosome ,with the help of dCas9 protein ,it will silence gene specificly.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None