Deleted
Sample Discontinued
Experience: None
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Part:BBa_K1550001

Designed by: Jesse Goldenberg   Group: iGEM14_BUGSS_Baltimore   (2014-10-17)


Metallothionein with RBS and tt

This part includes the strong RBS BBa_B0034 (Elowitz, 1999), BmtA from Oscillatoria brevis, which encodes lead binding metallothionein (Liu et al., 2003), and the double transcription terminator BBa_B0015. The BmtA sequence in this part has been codon optimized for E. coli. This part can be used with a constitutive or inducible promoter to produce metallothionein in projects utilizing the metal-binding protein for sequestration purposes.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 285
    Illegal AgeI site found at 300
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 250


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