Plasmid
pSB_LIC

Part:BBa_K155000

Designed by: Marc Slingerland, Nathan Puhl   Group: iGEM08_Lethbridge_CCS   (2008-10-25)

LIC plasmid

This is a plasmid intended for use in ligation-independent cloning. The overhang sequence is based on Aslanidis & DeJong (1990) "Ligation-independent cloning of PCR products (LIC-PCR)." Nucl. Acids Res. 18:20, 6069-6074.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1092


[edit]
Categories
Parameters
chassis
copies
mcs
originpMB1
resistanceA